The probiotic potential of Lactobacillus plantarum strain RW1 isolated from canine faeces.

Aim Evaluation of probiotic potential of Lactobacillus plantarum (L. plantarum) strain RW1 isolated from healthy dog for its further utilization as dietary supplement in dogs feeding METHODS AND RESULTS: The present study was aimed to evaluate the probiotic potential of L. plantarum strain RW1 (strain RW1) isolated from canine faeces. After confirming by conventional and then 16SrRNA sequencing, the identified strain RW1 was in vitro screened for its survivability in simulated gastrointestinal conditions, low pH, bile salts and adhesion to gut epithelial tissues, growth inhibitory effects on common pathogens and anti-inflammatory potential by measuring mRNA expression level of IL-6, IL-8, IL-1ꞵ in Salmonella infected MODE-K cells. Further, the effects on epithelial barrier function and host defensin peptide (beta-defensin 3) was checked by measuring mRNA expression level of tight junction protein (occludin) and beta-defensin 3 in MODE-K cells. The strain RW1 showed considerable potential to survive in simulated gastrointestinal environmental conditions, low pH, high bile salt concentrations along with good adhesion properties with MODE-K cell line. Pathogenic bacterial growth and their adhesion to MODE-K cell line was significantly inhibited by strain RW1. Real-time PCR analyses demonstrated that the strain RW1 inhibited Salmonella induced pro-inflammatory cytokines (IL-6, IL-8, IL-1ꞵ) production and reinforce expression of tight junction protein (occludin). The strain RW1 did not induce mRNA expression of beta-defensin 3. Conclusion Based on in vitro results the strain RW1 has potential to be used as a probiotic supplement in dogs feeding. However further study involving in vivo health effects is needed. Significance and impact of study Antibiotics have many side effects and nowadays the probiotics are considered potential antibiotics alternatives. This study evaluates the probiotic potential of dog isolated L. plantarum strain RW1 to use it as a dietary supplement in dogs feeding to control infectious diseases.