I NDICATORS IN SEDIMENT AND WATER SAMPLES AT A DMIRALTY BAY, KING GEORGE ISLAND, ANTARCTICA

Assessment of microbial faecal indicators was carried out in water and sediment samples of Admiralty Bay. Quantification of total coliforms, Escherichia coli , sulphite reducing clostridia and Clostridium perfringens was performed through the most probable number technique (MPN) using selective or differential media. Total coliforms were found in all sediment samples, but results may be due to false positive results as a consequence of adaptations in the method to increase its sensitivity. In water, positive results were found in EACF (CF) in all samples along the water column, and in one replicate from Botany Point, Thomas Point and Arctowski. E.coli results were positive only in CF sediment samples and in one Refuge 2 replicate, no E.coli was detected in water. The group of sulphide reducing clostridia showed to be widespread in Martel inlet and was also detected in Refuge 2, suggesting a possible influence of animal faeces in addition to human contribution. C. perfringens was detected in higher numbers in CF and Ullmann Point samples, but positive results were also observed at the other sites. The count values were variable between replicates, but results suggest that contamination in the CF area may persist to a small extent. Analysis of the microbial indicators in samples as animal faeces and a new assessment of the wastewater treatment system are necessary to provide lacking complementary information.