Ethanol metabolizing system in drosophila. Aldehyde dehydrogenase: Functional aspects in adult and during development

Abstract 1. 1. Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities were determined in adult flies from several Drosophila species endowed with widely different tolerance to ethanol (ETOH). 2. 2. Plotting ALDH against ADH activities resulted in a high correlation coefficient (r = 0.966). 3. 3. This finding was confirmed in developmental studies. From early larval stage up to late adult life, DH and ALDH activities demonstrated almost parallel profiles. 4. 4. In the highly ETOH tolerant species D. melanogaster (D.m.), ADH and ALDH profiles were U-shaped; high activities in larvae, low activities in pupae and high activities in adults. 5. 5. In D. simulans (D.s.), a species less tolerant to ETOH, the profiles were L- shaped; high activities in larvae but low activities in both pupae and adults. 6. 6. Interestingly, similar activities (ADH and ALDH) were observed in the larvae of both species. 7. 7. Subcellular distribution studies of larval ALDH in both species revealed that the total ALDH activity is largely contributed by a mitochondrial high affinity enzyme. 8. 8. ALDH activity, clearly distinguishable from aldehyde oxidase (ALDOX), was visualized through analytical isoelectric focusing of the subcellular fractions. The estimated pIs for D.m. and D.s. were 4.9 and 5.2 respectively, thus different from those of ADH. 9. 9. The key biological role initially attributed to Drosophila ALDH is further supported by the present data. In addition the Drosophila developmental model opens new avenues for research on the study of genetic regulation of ADH and ALDH expression.