Co-ordination betweeen Elongation and Division in Escherichia coli Mediated by the wee Gene Product

SUMMARY: A procedure to construct strains of Escherichia coli containing conditional lethal mutations in two different genes was used to construct ftsA-3(ts) we(Am) supF(ts) strain. This strain, OV-25-7, was used to ascertain whether the wee gene product (Wee) acts at the level of regulation of cell elongation or at the co-ordination of elongation and division. The mass per unit length and the buoyant density of cells in the absence of Wee increased only if division was allowed, as in the case of strain OV-25 (ww(Am) supf(ls)), but not when it was inhibited, as in strain OV-25-7. These results suggested that in E. coli the wee gene product was acting at the level of coordination between elongation and cell division.