Simultaneous determination of ginsenoside Rb1, naringin, ginsenoside Rb2 and oridonin in rat plasma by LC-MS/MS and its application to a pharmacokinetic study after oral administration of Weifuchun tablet.

Abstract A sensitive, specific and rapid liquid chromatography–mass spectrometry (LC–MS) method was developed and validated for analysis of ginsenoside Rb 1 , naringin, ginsenoside Rb 2 and oridonin in rat plasma using sulfamethoxazole as an internal standard (IS). Separation was conducted out on an Agilent Eclipse XDB C18 column with liner gradient elution using acetonitrile (A) and 0.1% aqueous acetic acid (B). A tandem mass spectrometric detection was conducted using multiple reaction monitoring (MRM) via an electrospray ionization (ESI) source. A novel multi-determination-periods program was executed to achieve a higher sensitivity by setting three scanning periods. All analytes exhibited good linearity within the concentration range ( r  > 0.9973). The lower limits of quantitation (LLOQ) of ginsenoside Rb 1 , naringin, ginsenoside Rb 2 and oridonin were 2.64, 4.32, 2.32 and 1.56 ng/mL, respectively. Intra-day and inter-day precisions of the investigated components exhibited an RSD within 8.3%, and the accuracy (RE) ranged from −8.6% to 6.0% at all quality control levels. The developed method was successfully applied to a pharmacokinetic study of ginsenoside Rb 1 , naringin, ginsenoside Rb 2 and oridonin in rats after oral administration of a Weifuchun tablet.