Partitioning of Plasmids in Streptomyces: Segregation in Continuous Culture of a Vector with Temperature-Sensitive Replication

Streptomycetes are not only important producers of nu­merous antibiotics and enzymes, but they are also of increa­sing interest as alternative bacterial hosts for the produc­tion of eukaryotic and other heterologous gene products. Recombinant vector plasmids with sufficient structural and segregational stability under non-selective conditions are necessary for this purpose.