Mechanical stretch enhances sex steroidogenesis in C2C12 Skeletal Muscle Cells

Abstract Skeletal muscle contains estrogens and estrogen synthesis-related enzymes. However, it has not been reported whether myoblasts cultured in vitro also express these enzymes. The purpose of the current study was to address these issues and to explore the effects of mechanical stretch on the enzyme system. The in vitro cultured C 2 C 12 mouse myoblasts were divided into the control, stretch, testosterone and stretch plus testosterone groups. Cells in the stretch and stretch plus testosterone groups were mechanically stretched with the Flexercell cell stress loading device at an amplitude of 10% and in a frequency of 0.5 Hz for 8 h. Cells in the testosterone and stretch plus testosterone groups were incubated with 100 nM testosterone for 24 h before distraction. Following the treatments, cell proliferation and estradiol levels, as well as the expressions of 17β-hydroxysteroid (17β-HSD), 3β-hydroxysteroid (3β-HSD) and aromatase were analyzed. Compared to the control, the cell proliferation in all experimental groups increased significantly, the estradiol levels in the mechanically stretched groups were significantly higher, and, moreover, the estradiol levels were positively correlated with the cell proliferation (r = 0.615, p  2 C 12 myoblasts cultured in vitro , the synthesized estrogen was closely related to the cell proliferation, and mechanical stretch was the external factor that affected the expression of the estrogen synthesis-related enzymes.