Efectos de la piridoxina en combinación con diferentes antioxidantes sobre parámetros de viabilidad y estrés oxidativo del semen caprino criopreservado

The effects of pyridoxine alone or combined with different antioxidants on viability and oxidative stress parameters of cryopreserved sperm of goat bucks were studied. Pooled semen samples diluted with tris-egg yolk extenders were supplemented in the Study I: with 0, 2, 4, 6 and 8 mM of pyridoxine; in the study II with 2 mM of pyridoxine (P) each combined with different concentrations (0, 2, 4, 6 and 8 mM) of vitamin E (PE), vitamin C (PC) and melatonin (PM); and in study III: with 2 mM of pyridoxine each supplemented with different combinations of vitamin C, vitamin E and melatonin (PCEM) each at different concentrations (0, 2, 4, 6 and 8 mM). The results showed higher (p<0.05) sperm motility in extenders supplemented with 4 mM and 6 Mm pyridoxine, or its combination in PM4 and PM6 followed by PC4, PC6 and PE4; and PCEM6 extenders. Extenders supplemented with pyridoxine alone or its combination at all levels of vitamins E, PM2, PCEM2, PCEM4 and PCEM8 had higher (p<0.05) acrosome integrity. Extenders supplemented with pyridoxine alone or in combination with all levels of vitamins E and PC2, PCEM2 and PCEM8 had higher (p<0.05) membrane integrity. Sperm abnormal and seminal leukocytes reduced (p<0.05) at 4 mM, 6 mM and 8 mM of pyridoxine or in combination with other antioxidants. Extenders supplemented with pyridoxine alone or combinations with antioxidants (PCEM4, PCEM6 and PCEM8) had reduced (p<0.05) malondialdehyde (MDA) concentrations. The findings indicated that extenders supplemented with pyridoxine alone or in combination with other antioxidants improved viability and reduced oxidative stress parameters of cryopreserved sperm of goat bucks.