Response of electrically stimulated cells of Pseudomonas oleovorans strain ATCC 29347 suspended in silicone oil
2001
A high intensity direct current was applied for more than 10 min onto a bacterial suspension of Pseudomonas oleovorans ATCC 29347 suspended in silicone oil. The application of a gradually increased electric field from 0 to 2500 V cm−1 resulted in a decrease of the optical density of the bacterial suspension and the occurrence of a peak current of several hundred μA for living cells instead of a linear increase (few μA) for killed or lyophilised cells. This procedure is not only a rapid way of investigating the living state of cell cultures but also an efficient experimental tool to study the cellular effects of a controlled electrical stress.
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