Fingerprint analysis of Radix Glycyrrhizae by fast HPLC

The objective of this paper is to develop a fast analysis method to determine fingerprints of Radix Glycyrrhizae from different areas of China for identification and quality control.The experiments were carried out under following conditions: Agilent Eclipse Plus C18(4.6 mm×50 mm,1.8 μm) column,acetonitrile and 0.05% phosphoric acid solution as the mobile phases with gradient elution,flow rate 1.0 mL·min-1,analysis time 11 min.The run time of the method was obviously decreased from 36 minutes to 11 minutes compared with routine HPLC method.The cluster analyses of the fingerprints of the 70 samples were performed by SPSS.The results showed that all samples were classified into 2 groups,59 Glycyrrhiza uralensis as well as 11 G.inflata.Three compounds,liquiritin apioside,liquiritin and glycyrrhiza acid should be considered as effective references for quality control of Radix Glycyrrhizae.This method can be used widely for identification and quality control of Radix Glycyrrhizae.