Crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, preparation method and application thereof

The invention discloses a crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, a preparation method and application thereof. 12 strains of antagonistic bacilli are respectively employed for mixed application with the cryptococcus laurentii, they all can improve the postharvest biocontrol effect of crystal pears, and four strains can reach the best effect. Finally, the application concentration and ratio of the antagonistic bacillus and cryptococcus laurentii for a best mixed application effect are determined. Every 1ml of a control agent contains 106-109 antagonistic bacilli and 106-109 cryptococcus laurentii, and the best control effect can be ensured when the bacterial number ratio is 1:1. The cryptococcus laurentii and antagonistic bacillus involved in the biological antistaling agent both have the characteristics of safety and high efficiency, and have the advantages of environmental friendliness, and outstanding biocontrol effects, etc. the mixed bacteria antistaling agent provided by the invention can further reduce the application of chemical bactericides, and has important significance to food safety, environmental protection, agricultural income increase and synergy, and sustainable social development, etc.