The expression characteristics of FBXW7 in human testis suggest its function is different from that in mice

Abstract F-box and WD domain protein 7 (FBXW7) is reported to bind with c-Myc in mouse spermatogonial stem cells, regulating self-renewal; however, the pattern and stage of expression of FBXW7 in human testes are unclear. In the present study, we examined the expression of human FBXW7 in adult testis, and analyzed fixed sections from adult testes and fetal testes to determine the cell type-specific expression pattern of FBXW7. The results showed that FBXW7α and FBXW7β genes are expressed in the testis; however, only FBXW7α protein could be detected. FBXW7 was not detected in human spermatogonial stem cells. Interestingly, FBXW7 was mainly expressed in the cell nuclei of later stage germ cells and differentiated somatic cells. We also observed high FBXW7 expression in human fetal germ cells, particularly in prespermatogonia. Our results raised the possibility that FBXW7 has different functions in humans and mice. The cell type-specific expression pattern of FBXW7 suggests that it performs regulatory functions during the late stage of human spermatogenesis instead of being involved in the self-renewal of spermatogonial stem cells.