Method for extracting antiviral active substance from phellinus igniarius

2010 
The invention discloses a method for extracting antiviral active substance from phellinus igniarius, which relates to a method for extracting active substance from traditional Chinese medicine. The method includes the following steps that: phellinus igniarius fruit bodies are dehydrated and ground under low temperature; ethyl acetate which is 2 to 10 times as large as the volume of the ground fruit bodies is added in the ground fruit bodies to carry out extraction, ultrasonic oscillation is carried out, filtrates are merged after filtration, and the filtrate is concentrated, frozen and dried into powder A. 90 percent of ethanol which is 2 to 10 times as large as the volume of filter residue extracted by the ethyl acetate is added into the filter residue to carry out extraction, ultrasonic oscillation is carried out, filtrates are merged after filtration, and the filtrate is concentrated, frozen and dried into powder B. Water with the temperature of 20 DEG C, which is 5 to 30 times as large as the volume of filter residue extracted by the ethanol is added in the filter residue, ultrasonic extraction is carried out, filtrates are merged after filtration, and the filtrate is concentrated, frozen and dried into powder C. According to the weight percentage ratio of 10 percent to 80 percent : 10 percent to 80 percent : 10 percent to 80 percent, the powder A, the powder B and the powder C are uniformly mixed. The obtained active substance has a good effect in resisting simian immunodeficiency virus (SIV), herpes virus (HSV) and enterovirus (EV71).
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