Drugs inducing T-cell mediated cutaneous adverse reactions and contact sensitizers evoke similar responses in THP-1 cells

Results All drugs significantly upregulated HMOX-1 gene (mean log2 values varying from 1.842 ± 0.164, p<0.01 for AMP to 3.096 ± 0.575, p<0.05 for OXP). All drugs, but the antiepileptics, increased the pro-inflammatory chemokine IL-8/CXCL8 (mean log2 values varying from 0.959 ± 0.154, p<0.05 for AMX and 4.729 ± 0.508, p<0.05 for OXP). Both DC maturations makers (CD83 and CD40) were significantly upregulated by VAP and AMP (respectively 1.594±0.672, p<0.05 and 0.999±0.226, p<0.05) and CD83 was also upregulated by VAP. Other genes studied were irregularly activated. Moreover, like DNFB, all drugs activated p38 MAPK. In general, allopurinol and oxypurinol showed the most intense effect, very similar to DNFB and inferior to LPS. The concomitant stimulation of THP-1 cells by OXP and AMP had no additive effect on the endpoints studied. Discussion