Characteristics and clinical significance of peripheral blood N-alpha-acetyltransferase 10 and lymphocyte immunophenotyping in patients with oral squamous cell carcinoma

Objective To analyze the characteristics of peripheral blood N-alpha-acetyltransferase 10 (Naa10) and lymphocyte immunophenotyping in patients with oral squamous cell carcinoma (OSCC) and to explore its clinical significance. Methods 97 OSCC patients and 50 health examinees were enrolled in the patient group and the control group respectively. Naa10 and lymphocyte immunophenotyping in the peripheral blood of the two groups were detected. The differences of Naa10 and lymphocyte immunophenotyping in the peripheral blood of patients with different TNM stages and lymph node metastasis status were compared, and their characteristics and clinical significance were summarized. Results The serum Naa10 in the patients group was (62.91±17.15) pg/ml, which was higher than that in the control group (38.82±9.04) pg/ml (t = 9.280, P 0.05) . There were 34 cases with lymph node metastasis, and the serum Naa10 was (65.15 ±14.07) pg/ml. There was no significant difference between those without lymph node metastasis and those without lymph node metastasis (61.45±12.66) pg/ml (t = 1.321, P > 0.05) . The percentages of CD3+, CD4+, CD4+/CD8+, NK cells in stageⅠ~Ⅱ patients were lower than those in stage Ⅲ~Ⅳ patients, and the percentages of CD8+, B lymphocyte in stageⅠ~Ⅱpatients were higher than those in stage Ⅲ~Ⅳ patients (P < 0.05) . Pearson correlation analysis showed that Naa10 in peripheral blood was negatively correlated with CD3+ (-0.631) , CD4+ (-0.529) , CD4+/CD8+ (-0.587) , percentage of NK cells (-0.603) , and positively correlated with CD8+ (0.558) and percentage of B lymphocyte (0.670) (P < 0.05) . Conclusion Naa10 in peripheral blood of OSCC patients was increased significantly, but not related to TNM stage and lymph node metastasis. The lymphocyte immunophenotype of OSCC patients changed significantly, and the increase of TNM stage and lymph node metastasis was accompanied by the decrease of CD3+, CD4+, CD4+/CD8+, NK cell percentage and the increase of CD8+, B lymphocyte percentage. Key words: Oral squamous cell carcinoma; N-alpha-acetyltransferase 10; Lymphocyte; Immunophenotyping