Fumigación con ácido acético y antimicrobianos para disminuir mortandad de Chrysoperla carnea por infección indeterminada

Chrysoperla carnea reproduced commercially by a laboratory (CREROB) acquired an infectious disease that kills larvae, pupae and adults. To counteract the infection, fumigation with acetic acid (AA) was evaluated in three doses and four times to eggs and four doses and six times to the pupae; the adult was given six different antimicrobial substances. Of the fumigated eggs of the laceworm, 50% of adults were obtained with respect to the non-fumigated eggs (p< 0.001). In contrast, adults from fumigated eggs survived twice as many as those not fumigated (p< 0.01). Some adults from fumigated eggs survived, but the fumigation did not prevent them from becoming infected and later the adult lacewings died. After 51 days after keeping the adults of lacewings with antimicrobial substances (in the water they drank), only cefotaxime at a dose of 1 200 μg ml-1 of active ingredient allowed to maintain no symptoms of infection and 100% survival. Chrysopters treated with antimicrobial substances survived between 50 and 81% while adults from fumigated eggs had a lower survival between 31 and 34%, possibly due to a greater asepsis of the former. Of the infected adult insects, two strains of Bacillus thuringiensis were isolated and their Cry toxins were characterized; although the lacewing infection is not attributed to B. thuringiensis, its presence is discussed.