Fermentation culture method for bacillus subtilis PTS-394

The invention relates to a fermentation culture method for bacillus subtilis PTS-394. The fermentation culture method comprises the following steps of: inoculating a bacillus subtilis PTS-394 seed solution in a fermentation culture medium and carrying out fermentation culture for two days on a shaking table. The fermentation culture method is characterized in that a carbon source in the fermentation culture medium is corn starch, and a nitrogen source is soybean powder; three key factors affecting bacterial content predicated value are corn starch, soybean powder and yeast powder; the content of the three key factors in the fermentation culture medium is 11.48g/L, 13.35g/L and 0.90g/L, respectively; three key factors affecting the bacterial content and the inhibition zone diameter in the fermentation culture conditions are temperature, an initial pH value and liquid filling amount; and in the fermentation culture process, the temperature is 30.84 DEG C, the initial pH value is 6.93 and the liquid filling amount is 65.15mL/250mL.