Plasmid control molecule for detection of transgenic soybean and building method thereof

The invention relates to a plasmid control molecule for detection of transgenic soybean and a building method thereof. The plasmid control molecule contains a control gene lectin specific sequence in soybean, a 3' end transformant specific sequence and a 5' end transformant specific sequence of transgenic soybean MON89788. Through designing a specific qualitative PCR primer, the lectin gene specific sequence, the 3' end transformant specific sequence and the 5' end transformant specific sequence of the MON89788 are obtained through amplification. Through molecular cloning techniques such as enzyme digestion, connection, transformation and the like, the three specific sequence segments are built into a plasmid molecule to obtain an artificially recombined plasmid control molecule pMD-LM3M5. According to tests, the plasmid control molecule built in the invention can fully substitute for the positive control of the transgenic soybean MON89788 and is suitable for the transformant specific qualitative and quantitative PCR analysis and detection of the MON89788 soybean.