Bioinformatics analysis of NRT gene sequences in Rhododendron fortunei

We used next generation sequencing technology to investigate the transcriptomes between inoculated roots and uninoculated roots of the Rhododendron fortunei and obtained many differentially expressed genes.In this paper,the nucleic acid sequences and amino acid sequences of nitrate transporters fromR.fortunei,were analyzed by bioinformatics tools.Several parameters of these sequences,including sequences composition,physicochemical property,leader peptide,topological structure of transmembrane regions,hydrophobicity or hydrophilicity,secondary structures,functional domains and protein structures,were predicted.Phylogenetic tree was reconstructed for the nitrate transporters protein family.Provide the bioinformatics foundation to understand NRTgene's function in inoculated seedling roots.