Vitamin C elicits the activation of immunological responses in swimming crab (Portunus trituberculatus) hemocytes against Mesanophrys sp.

Abstract Under suitable conditions, Vitamin C (vit. C) is essential for immunological responses and positively influences the function of stimulated immune cells. Therefore, this study evaluated the specific mechanisms of innate immunity in crab after supplementing the cell culture media with vit. C. We used the primary culture media of swimming crab hemocytes to detect some immune factors and immune-related signaling pathways via 24 h stimulation in vitro assays. Besides, we evaluated the anti-parasitic action of these hemocytes. Herein, the cellular and humoral factors, including intra- and extracellular phenol oxidase, nitrite oxidase synthase, lysozyme, and intracellular O2− were significantly (P