Alkaline protease and recombinant expression engineering bacterium thereof
2012
The invention relates to the technical field of microorganism engineering, in particular to alkaline protease and a recombinant expression engineering bacterium thereof. An alkaline protease gene of bacillus clausii is transferred into bacillus subtilis to construct a bacillus subtilis engineering strain with the preservation number of CCTCC (China Center for Type Culture Collection) No: M2012499. The engineering strain can express the alkaline protease efficiently, and the enzymatic activity of the engineering strain fermented in a 20L tank is as high as 9120U/ml. The recombinant expression alkaline protease can widely serve as an additive for a washing article.
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