Discussion of Chinese syndrome typing in acute hepatic failure model

Objective To study Chinese syndrome typing of acute hepatic failure (AHF) mice model by screening effective formulae. Methods Lipoplysaccharides (LPS)/D-galactosamine (D-GalN) was intraperitoneally injected to mice to establish the AHF mice model. Yinchenhao Decoction,Huanglian Jiedu Decoction,Buzhong Yiqi Decoction,and Xijiao Dihuang Decoction were administered to model mice respectively by gastrogavage. The behavior and the survival rate were monitored. The liver function and pathological changes of liver tissues were detected. Results In all the tested classic recipes,the survival rate was elevated from 10% to 60% by administration of Xijiao Dihuang Decoction. Five h after modeling,the serum alanine aminotransferase (ALT) level was (183.95±52.00) U/L,and aspartate aminotransferase (AST)(235.70±34.03) U/L in Xijiao Dihuang Decoction Group,lower than those of the model control group,but with insignificant difference (ALT:213.32±71.93 U/L; AST:299.48±70.56 U/L,both P0.05). Xijiao Dihuang Decoction could obviously alleviate the liver injury. Conclusions Xijiao Dihuang Decoction was an effective formula for LPS/D-GalN induced AHF model. According to syndrome typing through formula effect,heat toxin and blood stasis syndrome dominated in the LPS/D-GalN induced AHF mice model.