Cloning and Sequence Analysis of a Novel HMW-GS Gene 1Gy from Triticum araraticum Jakubz

In this study,genomic PCR reaction of Triticum araraticum(PI427305) was carried out by using degenerate primers specific for y-type HMW-GS.A 2.2 kb DNA fragment was amplified and cloned into the pMD18-T vector.DNA sequencing was carried out by using overlapping sub-clones.The complete coding region of the gene was 2 202 bp(GenBank accession number was HM131806),and 732 amino acid residues were encoded.It was found that the DNA sequence of this gene showed the highest identity with that of 1Gy7* of Triticum timopheevii Zhuk,and the corresponding amino acid sequence had the typical structure of y-type HMW-GS.So it was concluded that this gene was 1Gy.The molecular weight of 1Gy was a little smaller than that of 1Gy7*,and the mobility of 1Gy was slower than that of 1Gy7*.Therefore,1Gy was a novel y-type HMW-GS gene.Compared with other y-type subunits encoded by A,B and D genomes of common wheat,an extra cysteine existed in the repetitive domain near C-terminal domain of 1Gy.The repetitive domain was mainly proved to be coil by using PSIPRED.Both of them were beneficial to bread improvement.