Biochemical Evolution of DNA Polymerase η : Properties of Plant, Human, and Yeast Proteins

To assess how evolution might have biochemically shaped DNA polymerase η (Polη) in plants, we expressed in Escherichia coli proteins from Arabidopsis thaliana (At), humans (Hs), and the yeast Saccharomyces cerevisiae (Sc), purified them to near homogeneity, and compared their properties. Consistent with the multiple divergent amino acids within mostly conserved polymerase domains, the polymerases showed modest, appreciable, and marked differences, respectively, in salt and temperature optima for activity and thermostability. We compared abilities to extend synthetic primers past template cyclobutane thymine dimers (T[CPD]T) or undamaged T-T under physiological conditions (80–110 mM salt). Specific activities for “standing-start” extension of synthetic primers ending opposite the second template nucleotide 3′ to T-T were roughly similar. During subsequent “running-start” insertions past T-T and the next 5′ (N + 1) nucleotide, AtPolη and HsPolη appeared more processive, but DNA sequence contexts strongly af...