Temperature-Screening System for Determining Protein Crystallization Conditions

A newly proposed temperature-screening system has a practical application in determining protein crystallization conditions. Using a batch method, we demonstrated multitemperature conditions on one plate in a multitemperature experimental unit, providing accurate temperature control with a linear gradient. This screening system, which we call temperature at once (TAON), enables us to easily construct a solubility phase diagram and to search for the optimum temperature for crystal growth using microscale samples.