[Expression and significance of B cell-activating factor of TNF family (BAFF) and B cell lymphoma/leukemia-2 (BCL-2) in multiple myeloma].

This study was aimed to explore the expression of B cell-activating factor of TNF family(BAFF) and B cell lymyhoma/leukemia-2(BCL-2) in bone marrow mononuclear cells(BMMNC) of multiple myeloma(MM) and the significance of BAFF and BCL-2 for occurrence,development and prognosis of MM.The bone marrow of 40 cases of MM and 10 healthy persons was collected,the mononuclear cells(MNC) were isolated,the expression of BAFF and BCL-2 mRNA in BMMNC was detected by real-time PCR;the plasma was simultaneously collected and the β2-MG level was determined;the clinical staging of MM patients was performed according to Durie-Salmon(D-S) staging criterion.The results indicated that the expression level of BAFF and BCL-2 mRNA in MM patients increased,as compared with normal controls,the difference was statistical significant(p0.05);the expression level of BAFF and BCL-2 mRNA in plateau stage after treatment obviously decreased.The expression level of BAFF and BCL-2 mRNA in relapsed/refractory MM patients was significanty higher than that in normal controls and patients reached plateau stage(p0.05),there was no statistically significant difference between newly diagnosed and relapsed/refractory MM patients(p0.05).The expression of BAFF and BCL-2 mRNA related with D-S staging and β2-MG level.It is concluded that the expression levels of BAFF and BCL-2 mRNA increase,morever the expression levels of BAFF and BCL-2 mRNA in newly diagnosed and relapsed/refractory MM patients are higher than those in patients reached plateau stage,which suggest the BAFF and BCL-2 may be involved in occurrence and development of MM;the relation of expression level of BAFF and BCL-2 mRNA to MM load is positive,which indicates the expression level of BAFF and BCL-2 mRNA may be a new indicator for evatuating the prognosis of MM patients.