Polysaccharide-Coated Liposomal Amphotericin B for the Treatment of Murine Pulmonary Candidiasis

MIYAZAKI, T., KOHNO, S., SASAYAMA, K., INOUE, Y., HARA, K., OGASAWARA, M., SATO, T. and SUNAMOTO, J. Polysaccharide-Coated Liposomal Amphotericin B for the Treatment of Murine Pulmonary Candidiasis. Tohoku J. Exp. Med., 1992, 168 (3), 483-490 - Amylopectin-coated liposomal amphotericin B was investigated in a murine model of pulmonary candidiasis. The LD50 of amylopectincoated liposomal amphotericin B in normal mice was more than 10.0mg/kg, and that of conventional amphotericin B was 1.2mg/kg. Amylopectin-coated liposomes showed twice the concentration in the lungs of conventional liposomes. Candida albicans was inoculated intratracheally into BALB/C mice. Twenty- four hours later, the number of Candida in the lungs of mice treated with amylopectin-coated liposomes was less than in those treated with conventional liposomes, and amylopectin-coated liposomes improved the survival rate of inoculated mice. Coating liposomes with amylopectin aids the targeting of amphotericin B to the lungs. - liposome; amphotericin B; amylopectin; pulmonary candidiasis; HPLC