Evidence for activation of endothelium and monocytes in hypertensive rats.

We have proposed that an interaction between perivascular macrophages and endothelium via cytokines could underlie the increased risk of stroke in hypertension. Therefore, the activation of monocytes, the endothelial expression of intercellular adhesion molecule-1 (ICAM-1), and the numbers of monocytes/macrophages in carotid arteries, as well as the cytokine production in carotid tissue, of spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto and Sprague-Dawley rats were studied. The total number of blood monocytes (890 ± 153 cells/mm 3 , n = 10) and the number of activated (nitro blue tetrazolium-positive) monocytes (220 ± 51 cells/mm 3 , n = 10) were significantly greater (P < 0.05) in SHR than in WKY rats (440 ± 81 and 40 ± 16 cells/mm 3 , respectively, n = 10). Patchy endothelial expression of ICAM-1 was found in 77 ± 9% of carotid sections from stroke-prone SHR (SHR-SP, n = 5) and in 75 ± 7% of the sections from SHR (n = 7) but in none of the sections from the two normotensive rat strains (n = 7). The number of endothelium-attached monocytes/macrophages per millimeter of internal elastic lamina was significantly greater in SHR-SP than in SHR [5.1 ± 0.7 (n = 4) and 3.3 ± 0.3 (n = 6), P < 0.05], whereas no monocytes were found around the endothelium in either of the normotensive rat strains (n = 7 in each group). Incubation of the carotid arteries with lipopolysaccharide (30-300 ng/ml) induced a concentration-dependent expression of mRNAs for interleukin-1β and release of tumor necrosis factor-α to a significantly greater degree in the SHR than in the Wistar-Kyoto rats. The results demonstrate that hypertension is associated with activation of monocytes and endothelium and an increased endothelial adhesion and subendothelial accumulation of monocytes/macrophages and with an increased vascular capacity to produce cytokines.