Rapid high level production and purification of recombinant murine and human interferons alpha from Escherichia coli

The availability of large qualitities of pure interferon alpha (IFS-α) subtypes for in vivo studies has often proved difficult. This paper presents details on the use of the commercially available pGEX expression system for the production and purification of milligram (mg) quantities of recombinant Murine (Mu) and Human (Hu) IFSs-α-1 in Escherichia coli. Initially a fusion product is made which can be rapidly purified on a glulathione-sepharose 4B affinity matrix