Comparison of different cell disruption methods for Nostoc flagelliforme.

In order to get the best method for extraction of C-phycocyanin from Nostoc flagelliforme, the cell disruption of N.flagelliforme were investigated using five methods, including liquid nitrogen grounding, repeated freezing and melting, ultrasonic disruption, high-pressure homogenization, and glass beads-breaking treatment.And the five methods were compared by determination the breakage rate, extraction rate and purity of C-phycocyanin.Results showed that high-pressure homogenization was the most efficient method for cell disruption, with which the breakage rates of wild and liquid-cultured Nostoc flagelliforme reached 96.45% and 97.06%, respectively, when their cell density was 10 mg/mL.And repeated freezing and melting method was the most suitable method for extraction of C-phycocyanin from liquid-cultured Nostoc flagelliforme, with which the extraction rate and purity of C-phycocyanin were 1.28% and 0.468, respectively.For extraction of C-phycocyanin from wild Nostoc flagelliforme, high-pressure homogenization showed to be the best one.Using this method, the extraction rate and purity of C-phycocyanin were 1.43% and 0.153, respectively.