Immunosubtractive Electrophoresis on Gradient Polyacrylamide Gels

Abstract The specificity of antibodies was employed in the identification of bands separated by acrylamide gel electrophoresis by subtracting the band prior to the electrophoretic separation. This immunosubtraction was accomplished without sacrificing any of the high resolution obtained on gradient polyacrylamide gels. Using purified antibody (IgG) isolated by chromatography, the subtraction was performed in several ways. The utility of this technique was demonstrated on samples of human serum using antibodies against human transferrin, albumin, α2-macroglobulin, and whole human serum. Specific animal proteins added to the human serum samples were not subtracted.