Development and evaluation of a loop-mediated isothermal amplification method for the rapid detection of goose parvovirus.

By using PrimerExplorer V3 software,a series of specific primers were designed based on the nucleotide sequences of goose parvovirus(GPV)VP3 gene available in GenBank.These primers targeted 6 distinct conserved sequences of VP3 gene,and serve primers to perform a loop-mediated isothermal amplification(LAMP).These primers were used to establish rapid LAMP detection of GPV.The results showed the LAMP method specifically amplified GPV but not other common avian disease DNA viruses;The method was 100 times more sensitive than the conventional PCR method.The amplification could be finished within 1.5 h with good specificity and high sensitivity.With the addition of SYBR GREEN dye in the reaction mixture,the results could be differentiated by naked eyes.The results also proved that LAMP method is a reliable and accurate assay for rapid detection of goose parvovirus.This assay offers a new technique and alternative for detecting goose parvovirus.