Direct Observation of Solutes Trapping on a Surface-inert Reversed Phase Column.

The solute trapping process onto the surface-inert reversed phase packing was dirctly investigated with chromato-videoscope. The Fluorescence intensity of separating dansyl (DNS) amino acids in the column was detected by video camera and densitogarm of solutes along the column length was obtained with computer-processing of the video images. Band widths of trapped DNS amino acids were narrow in the absence of BSA in sample solution. However, in the presence of BSA, band shapes turned broad according to increase of the concentration of BSA or injected volume of the sample.