Clinical value of gene chip technology in the detection of genotyping and drug-resistant mutation of HBV

Objective To rapidly detect genotyping and drug-resistant mutation of HBV by means of gene chip technology.Methods Totally 211 serum samples were collected from the patients with chronic hepatitis B.The serum samples were detected by the FQ-PCR and DNA chip technology were adopted to detect the samples,and then the positive samples were further verified by DNA sequencing. Results FQ-PCR showed that the loads of HBV DNA were equal to or more than 5.0×102 IU/mL in the 211 samples.Totally 210 samples were gene chip assay positive;67 cases(31.9%) were detected as mutations in 210 cases.Among these positive samples detected by DNA chip,137 cases (65.2%) were genotype B,69 cases(32.9%) were genotype C,4 cases(1.9%) were mixed with genotype B and C.DNA sequencing confirmed the above results.Conclusion HBV Genotyping and Drug-resistant Mutations DNA chip technology is characteristic of accuracy,sensitivity and high-flux, and it can be used in HBV genotyping and drug-resistant mutantion detection.