Antioxidant, tyrosinase and urease inhibitory activities of camel αS-casein and its hydrolysate fractions

Abstract The aim of this study was to investigate the effect of pancreatic enzymes hydrolysis of camel αS-casein on their antioxidant, tyrosinase and urease inhibitory activities. Isolated αS-casein was hydrolyzed, fractionated and assayed for their antioxidant properties by using four complementary tests; namely, ABTS •+ scavenging, hydroxyl radical scavenging, ferrous ion chelating and β-carotene-linoleic acid assays. The in vitro enzymatic inhibitory activity was determined by using L-DOPA as a substrate and indophenol method for tyrosinase and urease inhibitory activity, respectively. After enzymatic hydrolysis, the activities were significantly increased whereas chymotryptic hydrolysis released peptides with higher activity than trypsin. The obtained fraction with low molecular weight (