離乳仔豬併欄時以Azaperone處理對血漿皮質醇與下視丘-腦下垂體-腎上腺軸緊迫相關基因表現變化之影響
2008
This study was conducted to investigate the effect of azaperone on the plasma cortisol and stress-related gene expression in the hypothalamus-pituitary-adrenal (HPA) axis alteration in co-mingling weaned pigs. Eighteen 28 days old piglets from 6 litters were allocated to 3 groups, S: co-mingled group, T: azaperone treatment group and C: piglets stayed beside their dams in the far- rowing house. Blood samples were collected prior and 2 hours after co-mingling to determine the plasma cortisol change. After the second blood sampling, all animals were electrically euthanized and the HPA tissues were quickly removed for target gene mRNA quantification by real-time RT-PCR. The results showed that the concentrations of cortisol in S group are significantly higher (P<0.05) than those in other groups. The gene expressions of hypothalamic c-fos in S and T group are down- regulated, and expressions of pituitary CRHR1 in S and T groups were up-regulated when compared to that in the C group. No significant differences on all these genes between S and T group were found. Thus, the co-mingling process was stressful to the weaned pigs, and azaperone may reduce the stress without influencing the stress-related gene expressions in the HPA axis.
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