Tissue culture and rapid propagation of pedicels of Hemerocallis hybrida.
2012
[Objective] This study aimed to establish tissue culture and rapid propagation methods for pedicels of Hemerocallis hybrida.[Method] Tender pedicels of dwarf H.hybrida were used as experimental materials to select callus induction,subculture and rooting medium for rapid propagation of H.hybrida.[Result] MS+2.0-3.0 mg/L of 6-BA+0.2-0.3 mg/L of NAA,MS+1.0-2.0 mg/L of 6-BA+0.1-0.2 mg/L of NAA,MS and 1/2MS+0.2 mg/L of NAA were the appropriate media for callus induction,subculture and rooting,respectively.[Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H.hybrida,which provide technical reference for the industrialized production of H.hybrida.
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