Gel Electrophoresis of Grapevine (Vitis vinifera L.) Isozymes - A Review

Several articles were written from the beginning of the fifties about the presence of plant enzymes in multiple forms. The major discussion was questioning whether these forms are artifacts that rose during the purification or not. To show that these forms are not artifacts in 1952 Jermyn divided his original peroxidase juice into two parts by acidic precipitation. The precipitate contained the A and B, while the supernatant the C and D points. Two components were found in the purified peroxidase solution; one migrated to the anode, the other to the cathode (Jermyn and Thomas, 1954).