Construction of bicistronic eukaryotic expression plasmid containing BMP-2 and TGF-β1 genes

Objective To construct a bicistronic eukaryotic expression plasmid consisting of BMP-2 and TGF-β1 target genes. Methods Firstly,the DNA fragments of 1188 bp BMP-2 and 1173 bp TGF-β1 gene were obtained from pGEM-T-BMP-2 and pGEM-T-TGF-β1 plasmids by PCR respectively. Then they were inserted into bicistronic eukaryotic expression plasmid vector plRES. The inserted target genes in the plasmid were verified by restriction enzyme digestion and nucleotide sequencing. Results The direction and sequences of the new bicistronic eukaryotic expression plasmid plRES-BMP-2-TGF-β1 with 1188 bp BMP-2 and 1173 bp TGF-β1 were correct. Conclusion The bieistronic eukaryotic expression plasmid is constructed successfully. Key words: BMP-2; TGF-β1; Eukaryotic expression vector