Trace element distribution in kidney of rats treated with CdCl2 by multivariable statistics and micro-SXRF

By using micro- scanning technique of SXRF (Synchrotron Radiation X-ray Fluorescence) combined with multivariable statistical analysis (correlation and hierarchi- cal cluster analysis), the positional distributions of eleanens in the kidney tissues of three groups of Wistar rats i.e, the control, that treated by CdCl2 and that Cd exposed then treated with a new complex) were obtained. The results show that Cd is mainly distributed in the renal cortex of rats treated by CdCl2, while. the level of Cd in the renal medulla is very low or not detectable. The elements measured in renal cortex of the normal rats can be classified into two groups and Cu, Zn., Mn and Se belong to the same group and have very strong co-locations. In acute CdCl2 poisoning, there is a very strong correlation between Cd and Se, and a secondly strong correlation between Cd and Ca. The classification is therefore different from that for the control. After administration of a new chelator, a very strong co-location and correlation can be observed between Zn and Se, and the classification of elements tends to return to the normal distribution.