Mailuoning prevents high-glucose-mediated human umbilical vein endothelial cells apoptosis

Abstract Objective To investigated the role of Mailuoning in the prevention of high-glucose-mediated cell apoptosis in human umbilical vein endothelial cells (HUVEC) and the mechanisms involved. Methods MTT assay was used to investigate cell viability, western blot was used to investigate protein expression, and flow cytometric detection technology was used to detect cell apoptosis. Results Exposure of HUVEC to high glucose (50 mM) significantly suppressed cell viability and increased cell apoptosis compared with normal glucose (11 mM) (all P Conclusion Mailuoning can prevent high-glucose-induced HUVEC apoptosis by suppressing p38 activation.