HPLC-based quality control of cytarabine preparations

An HPLC-based method for determining cytarabine (1-β-D-arabinofuranosylcytosine, Ara-C) and uracil arabinoside (Ara-U, a product of the hydrolytic deamination of Ara-C), as well other related compounds (cytidine, cyclocytidine, uridine), in aqueous solutions has been developed. The proposed method is characterized by good reproducibility and is capable of quantitatively determining all compounds simultaneously present in solution.