The construction of p53 gene knockout of plag1 transgenic mice with pleomorphic adenoma model

Objective:To construct the plag1 transgenic mice with p53 gene knockout model. Method:Two plag1+females were mated with one p53 heterozygote. All animals in this study were derived from these original founders so that both genotypes had the same mixed genetic background.F1 bred to generate plag1+p53-/-,plag+p53+/- and plag1+p53+/- genotypes. Measuring the size of the tumors within the mice of the same age for every two days. Comparing and Analysing the differences between the pleomorphic adenoma of those three different genotypes. Result:The mice with plag1+p53-/- genotype died early even before the tumors started to grow because of their gene defects。 The pleomorphic adenoma with plag+ p53+/- genotype grew faster than the ones with plag+ p53+/- genotypes with higher tendency of malignance. Conclusion:The p53 gene defection plays a role in the growth of pleomorphic adenoma of the plag1 transgenic mice.