Investigation of choline chloride-formic acid pretreatment and Tween 80 to enhance sugarcane bagasse enzymatic hydrolysis.

Abstract In this study, a pretreatment that consisting of choline chloride (ChCl) and formic acid (FA) were performed to improve sugarcane bagasse (SCB) enzymatic hydrolysis. Results showed that the ChCl-FA pretreatment exhibited an extraordinary ability to selectively extract hemicellulose (~95.6%) and degrade a large number of lignin (~72.6%) at 110 °C for 120 min, which enhanced the enzymatic hydrolysis of pretreated SCB. Besides, the impact of various additives on pretreated substrate enzymatic hydrolysis confirmed that Tween 80 was the best enzymatic additive, which could significantly improve the glucose produced from pretreated SCB and remarkably reduce the hydrolysis time (from 72 h to 48 h) and enzyme dosage (from 20 FPU/g pretreated solid to 10 FPU/g pretreated solid). In summary, the coupling of ChCl-FA pretreatment and Tween 80 exhibited a promising way to enhance the sugar release from SCB.