Study on optimization extraction of Bucleotide from beer yeast with RSM.

Using beer yeast as a raw material,the factors,such as,yeast concentration,time,pH,autolyzed temperature,and wheat root lixivium dosage were studied by single tests,then according to the Central Composite experimental design principles,these factors were analyzed with RSM,and the optimum conditions were obtained as follows,under 20% yeast latex,NaCl dosage 3.0%,pH6.6,autolyzed temperature 59 ℃,autolyzed time 12 h,then added wheat root lixivium 4.9%,processing 3 h at 60 ℃,the content of nucleotide in extraction liquid would reached over 0.34 g/100 mL.