Identification of Actinobacillus pleuropneumoniae biotype 1, serotype 1 from pig lungs with and without pneumonic lesions using an immunohistochemical technique.

IN THE PRESENT STUDY THE AVIDIN-BIOTIN-PEROXIDASE COMPLEX WAS USED FOR THE DETECTION OF ACTINOBACILLUS PLEUROPNEUMONIAE BIOTYPE 1, SEROTYPE 1, AND RESULTS WERE COMPARED WITH BACTERIAL ISOLATION. LUNGS FROM 100 CLINICALLY HEALTHY PIGS WERE COLLECTED AT A SLAUGHTERHOUSE, 50 HAD APPARENT PNEUMONIC LESIONS WHILE 50 HAD NO GROSS LUNG LESIONS. ALL SAMPLES WERE EXAMINED BY HISTOPATHOLOGICAL, BACTERIOLOGICAL AND IMMUNOHISTOCHEMICAL METHODS. PREDOMINANT MICROSCOPIC LESIONS WERE BRONCHOPNEUMONIA (30%) AND INTERSTITIAL PNEUMONIA (20%). THE IMMUNOHISTOCHEMICAL TECHNIQUE IDENTIFIED THE PRESENCE OF A. PLEUROPNEUMONIAE IN 75 LUNGS, WHEREAS BACTERIOLOGY ONLY DETECTED THIS MICROORGANISM IN 11 SAMPLES. STATISTICAL ANALYSIS SHOWED A SIGNIFICANT DIFFERENCE (P< 0.