Method for producing resveratrol by driving AhRESS genes through tobacco root specific promoter NtR2

2016 
The invention relates to a method for producing resveratrol by driving AhRESS genes through a tobacco root specific promoter NtR2 and belongs to the field of plant gene engineering. On the basis of cloning the tobacco root specific promoter NtR2 and the peanut AhRESS genes respectively, pBI121 is used as a parent carrier to construct a plant expression carrier pBI121-NtR2-AhRESS; then agrobacterium tumefaciens C58 is converted and tobaccos Cuibi No.1 are genetically transformed through a leaf-disc method to obtain transgenic tobacco plants; the transgenic tobacco plants are subjected to molecular identification and root specific expression function identification. The original tobaccos are genetically transformed by using rooting agrobacterium to mediate the pBI121-NtR2-AhRESS to obtain transgenic original tobaccos, so that the resveratrol only can be specifically expressed in original tobacco roots. The resveratrol in the transgenic original tobacco roots is extracted and is subjected to an HPLC (High Performance Liquid Chromatography) analysis; a result shows that the resveratrol is successfully produced in the transgenic original tobacco hairy roots.
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