Studies on the Production and Purification of Staphylococcal Enterotoxin B

By the method of cellophane covered agar plate culture, production of staphylococcal enterotoxin B was studied. After incubation at 37℃ for 48h, cells were harvested and the cell suspension was centrifuged at 10000×g for 15min at 4℃. The supernatant was condensed by polyglycol 20000. Then condensation was purified to electrophoretic homogeneity of the staphylococcal enterotoxin B by ion-exchange chromatography on CM-cellulose and by gel filtration on Sephadex G-200. This method was simple and could be applicable for laboratory.