Real-time PCR (polymerase chain reaction) non-diagnostic detection method of panda source rotavirus CH-1 strain

The present invention discloses a panda rotavirus Real-time PCR non-diagnostic method for detecting CH-1 strain, the upstream primer F: 5'-AGTTATACGCAGCACGGACC-3 ', downstream primer R: 5'-GTTGTTGCGTTTGGTGTGGT-3; primer concentration was 0.5μmol / L, Real-time PCR cycling conditions: 95 ℃ denaturation 30s, 94 deg.] C denaturation 5s, 60.5 ℃ annealing 30s, for 40 cycles, fluorescent signal is detected after each cycle. The minimum number of copies fluorescence quantitative RT-PCR method of the present invention establishes detected was 1.0 × 100 copies / μL, 100 times more sensitive than the conventional RT-PCR least.