Optical monitoring of the meningeal lymphatic clearance after opening of blood-brain barrier

In our experiments on rats we performed visualization of brain clearing from substances injected into the brain parenchyma using optical coherent tomography and fluorescence microscopy. Our results uncovered that the meningeal lymphatic system play an important role in the brain clearing processes, where the deep cervical lymph node is the first anatomical station of cerebral fluid exist from the brain. These data shed light on the novel mechanisms underlying drainage and clearing function of the brain.